Anti-NMDAR2A Antibody (C15-1315-793)

General description

NMDA (N-methyl-D-aspartate) receptors are a class of ionotropic glutamate receptors. The NMDAR channel has been shown to be involved in long term potentiation, an activity dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of the key receptor subunit NMDAR1 (GRIN1) and 1 or more of the 4 NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D).

Specificity

NMDAR2A. By Western blot it recognizes a 180 kDa band in rat brain membranes. Does not react with NMDAR2B or NMDAR2C. Immunolabeling is blocked by preadsorption of antibody with the immunogen.

Immunogen

C-Terminal Fusion Protein of rat NMDAR2A, aa 1253-1391.

Epitope: C-terminus

Application

Anti-NMDAR2A Antibody is an antibody against NMDAR2A for use in IH, IP & WB.

Immunoprecipitation:
3 μg of a previous lot was used to (under appropriate conditions) quantitatively immunoprecipitate all NMDAR2A in 200 μg of rat brain.

Immunohistochemistry:
A previous lot of this antibody was used at 1:1,000-1:2,000 dilution.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurotransmitters & Receptors

Quality

Routinely evaluated by Western Blot on Rat Brain Microsomal lysates.

Western Blot Analysis: 1:500 dilution of this lot detected NMDAR2A on 10 μg of Rat Brain Microsomal lysates.

Target description

180 kDa

Physical form

ImmunoAffinity Purified

Purified rabbit polyclonal lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservative.

Storage and Stability

Lyophilized material stable for 1 year at -20°C from date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up 6 months.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
NMDAR2A is present in high concentrations in the hippocampus.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human, mouse, fish. manufacturer/tradename: Chemicon^®. . technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_000824. UniProt accession no.: Q12879. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... GRIN2A(2903). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1.