ANTI-NQO1 CLONE 1D12 ZOOMAB RABBIT MONOCLONAL (C15-1670-160)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D12 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects NQO1. It targets an epitope within 25 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human NAD(P)H dehydrogenase [quinone] 1 (NQO1).

Application

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected NQO1 in HepG2 cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected NQO1 in LNCap cells and in Mouse and Rat Liver lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NQO1 in human stomach tissue sections.

Immunocytochemistry Analysis: A 1:300 dilution from a representative lot detected NQO1 in HepG2 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

NAD(P)H dehydrogenase [quinone] 1 (UniProt: P15559; also known as EC:1.6.5.2, Azoreductase, DT-diaphorase, DTD, Menadione reductase, NAD(P)H:quinone oxidoreductase 1, Phylloquinone reductase, Quinone reductase 1, QR1, NQO1) is encoded by the NQO1 (also known as DIA4, NMOR1) gene (Gene ID: 1728) in human. NQO1 is a highly inducible, homodimeric, FAD containing quinone reductase that catalyzes the 2-electron reduction of a broad range of quinones and plays a role in cellular adaptation to stress. It serves as a quinone reductase in connection with conjugation reactions of hydroquinons involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent g-carboxylation of glutamate residues in prothrombin synthesis. It can accept electrons from both NADH and NADPH with equal efficiency. Its activity can be induced by oxidative stress and offers protection against redox cycling and reactive oxygen species. It also protects a wide range of proteins, including p53, from proteasomal degradation. NQO1 is shown to be expressed at higher levels in epithelial tissues, vascular endothelium, and adipocytes. It is also expressed at higher levels in many solid tumors. It has been shown to stabilize many proteins, including the p53, by inhibiting their proteasomal degradation. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Ross, D., and Siegel, D. (2017). Front. Physiol. 8; 595; Siegel, D., et al. (2012). PLoS ONE 7(9); e44861).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.