Anti-NR1D1 (Rev-ErbA alpha) Antibody (C15-1315-626)

General description

Rev-ErbA alpha (NR1D1 or nuclear receptor subfamily 1, group D, member 1), is a member of the Rev-ErbA family of nuclear receptors and is a transcriptional repressor. In mammals, Rev-Erbα is highly expressed in the liver, skeletal muscle, adipose tissue, and the brain, participating in the development and circadian regulation of these tissues. Rev-Erbα regulates gene transcription by directly binding to target response elements (RevREs), comprises an A/T-rich flank followed by AGGTCA. Rev-Erbα mediates repression by recruiting the corepressor N-CoR, which then activates the histone deacetylase (HDAC) 3. A number of target genes has been identified for Rev-Erbα, including the lipoproteins ApoA1 and ApoCIII, hydratase dehydrogenase, the circadian factor BMAL, and the anti-fibrinolytic factor PAI-1. Many of these genes are coordinately regulated by Rev-erbα and the RAR-related orphan receptor RORα, which share the same response elements but exert opposite effects on gene transcription. Crosstalk between Rev-Erbα and RORα likely acts to fine-tune of their target physiologic networks, such as circadian rhythms, metabolic homeostasis, and inflammation.

Specificity

Mouse and rat (92% sequence homology).

This antibody recognizes NR1D1 near the N-terminus.

Immunogen

Epitope: Near N-terminus

KLH-conjugated linear peptide corresponding to residues surrounding the area near the N-terminus region of NR1D1.

Application

Anti-NR1D1 (Rev-ErbA α) Antibody is a Rabbit Polyclonal Antibody for detection of NR1D1 (Rev-ErbA α) also known as V-erbA-related protein EAR-1 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected NR1D1 in 10 µg of A431 cell lysate.

Target description

~67 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide.

Storage and Stability

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): western blot: suitable. NCBI accession no.: NP_068370. UniProt accession no.: P20393. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... NR1D1(9572). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.