Anti-NR2F2 (ARP-1) isoform B (C15-1317-043)

General description

COUP transcription factor 2 (UniProt: P24468-2; also known as COUP-TF2, COUP-TF II, Apolipoprotein A-I regulatory protein 1, ARP-1, COUP transcription factor II, COUP-TF II, Nuclear receptor subfamily 2 group F member 2) is encoded by the NR2F2 (also known as ARP1, TFCOUP2) gene (Gene ID: 7026) in human. Coup-TF II is a ligand-activated transcription factor that is activated by high concentrations of 9-cis-retinoic acid and all-trans-retinoic acid. Its DNA binding region is localized in amino acids 76-151. COUP-TF II contains two NR C4-type zinc finger domains (aa 79-99 and 115-139). It is widely distributed in human tissues with detectable expression in all tissues examined. Its tissue and cell-specific expression can be modulated both transcriptionally and post-translationally. Through binding to 5 -AGGTCA-3 direct repeats (DRs) with variable spacing, it can modulate the expression of target genes, such as RA receptor beta 2, PEPCK, NGFI-A, and cholesterol 7 -hydroxylase. COUP-TF II action can also be enhanced by interaction with certain coactivators, such as with members of the steroid receptor coactivator family members SRC-1, SRC-2, and SRC-3. Mutations in NR2F2 gene have been linked to multiple types of congenital heart defects characterized transposition of the great arteries, aortic stenosis, and ventricular septal defect. (Ref.: Litchfield, LM and Klinge, CM (2012). J. Mol. Endocrinol. 49 (3); R135-R148).

Specificity

This rabbit polyclonal antibody detects COUP-TF II. It targets an epitope within 14 amino acids from the N-terminus region of isoform B.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the first 14 amino acids from the N-terminus region of human COUP transcription factor 2 (ARP1), isoform B.

Application

Anti-NR2F2 isoform B, Cat. No. ABE2586, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 2 group F member 2. and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 2 µg/mL from a representative lot detected NR2F2 isoform B in in 10 µg of L6 cell lysate.

Western Blotting Analysis: A representative lot detected NR2F2 isoform B in MEWO cells overexpressing NR2F2 isoform B.(Courtesy of Claudia Lovell at Hernando Lab, NYU Langone Medical Center).

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected NR2F2 isoform B in human kidney, human pancreas, and human cerebellum tissues.

Quality

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NR2F2 isoform B in in 10 µg of PC12 cell lysate.

Target description

~37kDa observed; 31.48 kDa calculated for isoform B. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat. species reactivity (predicted by homology): mouse (based on 100% sequence homology), sheep (based on 100% sequence homology), human (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable. isotype: IgG. NCBI accession no.: NP_001138627. UniProt accession no.: P24468-2. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... NR2F2(7026). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.