General description
Cell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.
Immunogen
Human nuclei
Application
Evaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in H9 human embryonic stem cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected nuclei in H9 human embryonic stem cells. Evaluated by Immunocytochemistry in mouse embryonic fibroblasts. Immunocytochemistry Analysis: A 1:50 dilution of this antibody did not detect nuclei in mouse embryonic fibroblasts.
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Developmental Neuroscience
Mesenchymal Stem Cells
Neurodegenerative Diseases
The anti-Nuclei, clone 235-1, Cy3 conjugate will recognize human cell nuclei only & is usefull in xenograph transplantation studies to study stem cell migration.
Quality
Evaluated by Immunocytochemistry in human foreskin fibroblasts (SCC058). Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected nuclei in human foreskin fibroblasts (SCC058).
Physical form
Protein G Purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15mg/ml BSA.
Storage and Stability
Maintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
Analysis Note
Control
Human foreskin fibroblasts (SCC058)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 41181506
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB1281C3
- Temperature Control Device:
- Yes
