ANTI-NUDT21 (C005B-240910)

General description

Cleavage and polyadenylation specificity factor subunit 5 (UniProt: O43809; also known as Cleavage and polyadenylation specificity factor 25 kDa subunit, CFIm25, CPSF 25 kDa subunit, Nucleoside diphosphate-linked moiety X motif 21, Nudix motif 21, Pre-mRNA cleavage factor Im 25 kDa subunit) is encoded by the NUDT21 (also known as CFIM25, CPSF25, CPSF5) gene (Gene ID: 11051) in human. CFlm25 is a homodimeric protein that is a component of the cleavage factor Im (CFIm) complex, which plays a key role in pre-mRNA 3′-processing. It is involved in association with CPSF6 or CPSF7 in pre-MRNA 3′-end poly(A) site cleavage and poly(A) addition. The interaction of CFlm25 with the RNA is one of the earliest steps in the assembly of the 3′ end processing complex and facilitates the recruitment of other processing factors. It contains one Nudix hydrolase domain (aa 76-201). The region containing aa 2-147 is considered to be essential for RNA-binding. It can be acetylated by p300/CBP and acetylation results in a reduction in its interaction with Poly(A) polymerase alpha (PAPAO).

Specificity

This rabbit polyclonal antibody detects Cleavage and polyadenylation specificity factor subunit 5 (CFIm25) in human cells.

Immunogen

Epitope: unknown

His-tagged full length recombinant human Cleavage and polyadenylation specificity factor subunit 5 (CFIm25).

Application

Anti-NUDT21, Cat. No. ABE2873, is a highly specific rabbit polyclonal antibody that targets Cleavage and polyadenylation specificity factor subunit 5 and has been tested in Immunoprecipitation and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected NUDT21 in RNase A-treated HeLa whole-cell extract after IP (Katahira, J., et. al. (2013). Nucleic Acid Res. .41(14):7060-72).

Immunoprecipitation Analysis: A representative lot detected NUDT21 in Co-immunoprecipitation with FLAG-tagged CF I cell lysates (Shimazu, T., et. al. (2007). J Biol Chem. 282(7):4470-8).

Quality

Evaluated by Western Blotting in Caco-2 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NUDT21 in 10 µg of Caco-2 cell lysate.

Target description

~25 kDa observed; 26.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. packaging: antibody small pack of 25 . μ. L. technique(s): immunoprecipitation (IP): suitable, western blot: suitable. isotype: IgG. NCBI accession no.: NP_008937. UniProt accession no.: O43809. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... NUDT21(11051). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.