ANTI-NUR77/NR4A1 CLONE 1F2 ZOOMAB« RABBIT MONOCLONAL (C005B-260975)

General description

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1F2 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Nur77/NR4A1. It targets an epitope within 17 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal half of human Nur77/NR4A1.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected Nur77/NR4A1 in HeLa cell lysate.

Tested Applications

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Nur77/NR4A1 in Human liver tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Nur77/NR4A1 peptide with a KD of 2.4 x 10-6 in an affinity binding assay.

Enzyme Immunoassay Analysis (ELISA): A serial of dilutions from a representative lot detected recombinant Human Nur77/NR4A1.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

NuCLEAR^™ receptor subfamily 4 group A member 1 (UniProt: P22736; also known as Early response protein NAK1, NuCLEAR^™ hormone receptor NUR/77, Nur77, Orphan NuCLEAR^™ receptor HMR, Orphan NuCLEAR^™ receptor TR3, ST-59, Testicular receptor 3) is encoded by the NR4A1 (also known as GFRP1, HMR, NAK1) gene (Gene ID: 3164) in human. Nur77 is an orphan NuCLEAR^™ receptor that may ACT^® concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Its expression is observed in fetal muscle and in adult liver, brain, and thyroid and is reported to be induced by growth stimulating agents. It binds the NGFI-B response Element^™ (NBRE) 5′-AAAAGGTCA-3′. Its DNA binding region is localized to amino acids 264-339. It contains two zinc finger domains (aa 267-287 and 303-327). Nur77 is shown to heterodimerize with retinoid X receptor alpha and is actively transported from the nucleus to the mitochondria in cells undergoing apoptosis. It binds to Bcl-2 and triggers a conformational change that exposes the pro-apoptotic BH3 domain. This converts Bcl-2 from an anti-apoptotic to a pro-apoptotic factor. In several cancers the NuCLEAR^™-mitochondrial translocation of Nur77/TR3 is defective, which prevents apoptosis. Three isoforms of Nur77 have been described that are produced by alternative splicing. It is acetylated by p300/CBP that increases its stability. It can also undergo phosphorylation at Serine 351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.  This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, Affinity^™, reproducibility, and stability over conventional monoclonals. (Ref.: Liu, J., et al. (2017). Oncotarget. 8(41); 69731-69745; Lin, B., et al. (2004). Cell. 116(4); 527-540).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ACT is a registered trademark of My Green Lab, Corp. non-profit corporation

Affinity is a trademark of Mine Safety Appliances Co.

Element is a trademark of Corning, Inc.

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.