General description
2′-5′-oligoadenylate synthase 1 (EC 2.7.7.84; UniProt P00973; also known as (2-5′)oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS) is encoded by the OAS1 (also known as OIAS, OIASI) gene (Gene ID 4938) in human. Pattern recognition receptors (PRRs) recognize pathogen-associated molecular patterns (PAMPs), including pathogens-derived double-stranded RNA and DNA (dsRNA and dsDNA). The 2′-5′ oligoadenylate synthetases (OAS) are interferon-induced cytoplasmic PRRs that recognize virally produced dsRNA and initiate RNA destabilization through activation of RNase L within infected cells. Upon dsRNA binding, OAS enzymes generate the second messenger 2’-5’ oligoadenylate (2-5An; n = 2 to 19), resulting in the dimerization and activation of the latent ribonuclease (RNase L) that degrades host and viral mRNAs. In primates, the OAS family consists of OAS1, OAS2, OAS3, and the catalytically inactive OASL, while 12 rodent Oas genes have been identified, eight of which are OAS1 paralogs.
Specificity
Clone 1D11.1 was raised against a recombinant fragment derived from the spliced isoform p46 (E18; Uniprot P00973-1), cross-reactivity toward the p41 (E16; 3-9), p48 (9-2), and p44 spliced isoforms is possible, but has not been determined.
Immunogen
Epitope: Internal (C-terminal half).
GST-tagged recombinant internal fragment from the C-terminal half of human OAS1.
Application
Anti-OAS1 Antibody, clone 1D11.1 is an antibody against OAS1 for use in Western Blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
RNA Metabolism & Binding Proteins
Quality
Evaluated by Western Blotting in lysate of IFN gamma-treated HeLa cells.
Western Blotting Analysis: A 1:500 dilution of this antibody detected OAS1 in 10 µg of lysate from IFN gamma-treated HeLa cells.
Target description
~50 kDa observed. 46.03 kDa (isoform p46; E18), 41.74 kDa (isoform p41; E16; 3-9), 47.41 kDa (isoform p48; 9-2), and 43.94 kDa (isoform p44) calculated.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 51373449
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MABC943
- Temperature Control Device:
- Yes
