Anti-OFD1 Antibody (C15-1316-793)

General description

The cilium is a cell surface projection found in many vertebrate cells and it is required to transduce signals important for development and tissue homeostasis. OFD1 is important in cilium development, and thus it is important in cellular development and signaling. OFD1 is a component of the centrioles and appears to control the length of the centrioles in mother-daughter cells. OFD1 also plays an important role in the WNT signaling pathway and in the formation of the left-right axis in embryos. OFD1 is expressed in the cytoplasm during development in most tissues but can be found in the nucleus as well, and it is known to interact with proteins important in chromatin remodeling as well. At least one X-linked genetic mutation is associated with defects in the OFD1 gene. It is a form of oroffaciodigital syndrome characterized by abnormalities in the oral cavity, face, digits and other disfigurements that is lethal in males.

Immunogen

Epitope: C-terminus

Linear peptide corresponding to the C-terminus of mouse OFD1.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-OFD1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of OFD1.

Western Blotting Analysis: A representative lot detected OFD1 in untreated and autophagy-induced/serum-starved mouse MEFs (Tang, Z., et al. (2013). Nature. 502:254-257).
Western Blotting Analysis: A representative lot detected OFD1 in wild-type female mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Western Blotting Analysis: A representative lot detected OFD1 in male E10.5 knock-out mouse embryo lysates (Ferrante, M.I., et al. (2006). Nat Genet. 38(1):112-117).
Immunocytochemistry Analysis: A representative lot detected OFD1 in PCM1 knockdown U2OS cells & untreated and serum-starved/autophagy-induced U2OS cells where OFD1 partially colocalizes with LC3 (Tang, Z., et al. (2013). Nature. 502:254-257).

Quality

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected OFD1 in 10 µg of U2OS cell lysate.

Target description

~120 kDa observed. Uncharacterized bands may be observed in some lysates at 45 kDa, 33 kDa & 28 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_803178. UniProt accession no.: Q80Z25. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: rat ... Ofd1(302661). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.