Anti-Olig-2 Antibody, Alexa Fluor 488 Conjugate (C15-1316-543)

General description

Oligodendrocyte transcription factor 2 (UniProt Q9EQW6; also known as bHLHe19, Olig-2, Oligo2, RACK17, RK17) is encoded by the Olig2 (also known as Bhlhb1) gene (Gene ID 50913) in murine species. Olig2 is a basic helix-loop-helix (bHLH) transcription factor expressed in a spinal cord ventricular zone domain where it mediates the sequential generation of motoneurons and oligodendrocytes during embryogenesis. Olig2 also functions as an anti-neurogenic factor in pMN progenitors at early time points to sustain the cycling progenitor pool. The anti-neurogenicity of Olig2 is also responsible for its role in tumorigenesis, especially in brain tumors and leukemia. Due to its expression in glioblastoma and other diffuse gliomas, Olig2 is a useful diagnostic marker of these brain tumors.

Specificity

The unconjugated antibody (Cat. No. AB9610) recognizes the ~32 kDa Olig-2 protein by Western blotting.

Immunogen

Recombinant mouse Olig-2.

Application

Anti-Olig-2 Antibody, Alexa Fluor^™ 488 Conjugate is an antibody against Olig-2 for use in Immunofluorescence.

Research Category
Neuroscience

Research Sub Category
Transcription Factors

The unconjugated antibody (Cat. No. AB9610) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications.

Quality

Evaluated by Immunofluorescence in mouse brain tissue.

Immunofluorescence Analysis: A 1:50-100 dilution of this antibody detected Olig-2 in mouse brain tissue sections.

Target description

~32 kDa observed.

Physical form

Ammonium Sulfate

Purified rabbit polyclonal antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. conjugate: ALEXA FLUOR^™. 488. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, mouse, human. technique(s): immunofluorescence: suitable. NCBI accession no.: NM_016967.2. UniProt accession no.: Q9EQW6. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... OLIG2(10215). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.