Anti-Olig-2 Antibody (C15-1317-650)

General description

Oligodendrocyte transcription factor 2 (UniProt Q9EQW6; also known as bHLHe19, Olg-2, Oligo2, RACK17, RK17) is encoded by the Olig2 (also known as Bhlhb1) gene (Gene ID 50913) in murine species. Olig2 is a basic helix-loop-helix (bHLH) transcription factor expressed in a spinal cord ventricular zone domain where it mediates the sequential generation of motoneurons and oligodendrocytes during embryogenesis. Olig2 also functions as an anti-neurogenic factor in pMN progenitors at early time points to sustain the cycling progenitor pool. The anti-neurogenicity of Olig2 is also responsible for its role in tumorigenesis, especially in brain tumors and leukemia. Due to its expression in glioblastoma and other diffuse gliomas, Olig2 is a useful diagnostic marker of these brain tumors.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence in the N-terminal region of mouse Olig-2.

Application

Anti-Olig-2 Antibody is an antibody against Olig-2 for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry (Paraffin).

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Olig-2 in 500 µg of mouse brain tissue lysate.
Immunohistochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in rat hindbrain tissue sections.
Immunocytochemistry Analysis: An 1:500 dilution from a representative lot detected Olig-2 in PC12 cells.

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Olig-2 in 10 µg of mouse brain tissue lysate.

Target description

~37 kDa observed. 32.40 kDa calculated.

Linkage

Replaces: AB15328

Physical form

Purified rabbit polyclonal antibody in PBS and 0.1 % sodium azide.

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_058663. UniProt accession no.: Q9EQW6. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... OLIG2(10215). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.