ANTI-OLIGODENDROCYTES ANTIBODY CLONE CE-1 (C005B-452491)

General description

Oligodendrocytes are large glial cells found in the central nervous system. They are responsible for production of myelin sheath that insulates neuronal axons. The first step in the generation of oligodendrocytes is the emergence of oligodendrocyte precursors (OPCs) from embryonic radial glia. Subsequently, OPCs differentiate into immature oligodendrocytes before they begin to generate myelin. This process is regulated by both intrinsic and extrinsic signals in cells. Oligodendrocytes have only a brief period for myelination early during differentiation, and mature oligodendrocytes are incapable of myelinating. An essential signal for the onset of myelination is reported to be provided by the electrical activity of neurons. Although the ATP released from axons firing axon potentials does not directly act on OPCs or oligodendrocytes, it triggers the release of leukemia inhibitory factor (LIF) from astrocytes, which promotes myelination. Although the electrical activity of neurons is an essential promyelinating factor, other factors also come into play in this process. During development, all growing nerve fibers are shown to express the polysialylated-neural cell adhesion molecule (PSA-NCAM) that can prevent homophilic NCAM adhesion and cell-cell interactions. When this form of NCAM is downregulated, as it is in electrically active neurons, only then the process of myelination can proceed. (Ref.: Bradl, M., and Lassmann, H. (2010). Acta Neuropathol. 119(1); 37-53; Berto, S., et al. (2019). Proc. Natl. Acad. Sci. USA. 116(48); 24334-24342).

Specificity

Clone CE-1 is a mouse monoclonal antibody that detects oligodendrocytes.

Immunogen

Rat glial cell membrane proteins followed by rat CNS white matter.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in Rat oligodendrocytes.

Immunocytochemistry Analysis: A 1:250 dilution of this antibody detected Rat Oligodendrocytes.

Tested Applications

Western Blotting Analysis: A representative lot detected Oligodendrocytes in Western Blotting application (Goolsby, J., et al. (2003). Proc Natl Acad Sci USA. 100(25):14926-31).

Immunocytochemistry Analysis: A representative lot detected Oligodendrocytes in Immunocytochemistry application (Goolsby, J., et al. (2003). Proc Natl Acad Sci USA. 100(25):14926-31; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81).

Immunofluorescence Analysis: A representative lot detected Oligodendrocytes in Immunofluorescence applications (Dubreuil, C., et al. (2003). J Cell Biol. 162(2):233-43; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81; Wuthrich, C., et al. (2012). J Neuropathol Exp Neurol. 71(1):54-65).

Immunohistochemistry Applications: A representative lot detected Oligodendrocytes in Immunohistochemistry applications (Dubreuil, C., et al. (2003). J Cell Biol. 162(2):233-43; Glavaski-Joksimovic, A., et al. (2009). Neuroscience. 162(2):472-81; Wuthrich, C., et al. (2012). J Neuropathol Exp Neurol. 71(1):54-65).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Physical form

Purified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.

Reconstitution

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.