Anti-Orai1 Antibody, Intracellular (C15-1316-593)

General description

TRP (transient receptor potential)-related ion channels (TRPCs) have been postulated as candidates for forming both receptor-operated (ROCE) and capacitative Ca2+ entry channels across the plasma membrane. ER transmembrane protein STIM1 senses the depletion of Ca2+ from ER stores. Orai, a regulatory β-subunit of the TRPC ion channel α-subunit, transduces the STIM1 signal into activation of the TRPC. Thus, Orai proteins by interacting with TRPCs act as regulatory subunits that confer STIM1-mediated store depletion sensitivity to these channels and regulate Ca2+ influx through the plasma membrane.

Specificity

ORai1, intracellular. Detects both the glycosylated and unglycosylated forms of ORai1. By Western blot the antibody reacts with the ~ 50 kDa ORai1 protein. Additional bands between 35 and 50 kDa may be seen if analyzing overexpressed ORai1. These additional bands most likely represent different degrees of glycosylation of ORai1

Immunogen

Synthetic Peptide from the Intracellular domain of Orai1

Application

Immunoblotting: 1:1,000-1:5,000
Immunofluorescence: 1:1,000-1:2,500
Immunoprecipitation: 1 µg
Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Growth Cones & Axon Guidance

This Anti-Orai1 Antibody, Intracellular is validated for use in WB for the detection of Orai1.

Target description

50 Kda

Physical form

Affinity purified immunoglobulin in PBS with 0.1% NaN3

Format: Purified

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. UniProt accession no.: Q96D31. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ORAI1(84876). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.