MONOCLONAL ANTI-ORAI1 ANTIBODY PRODUCED IN MOUSE CLONE ORAI1-89 PURIFIED IMMUNOGLOBULIN

General description

Monoclonal Anti-Orai1 (mouse IgG1 isotype) is derived from the hybridoma ORAI1-89 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. Orai1, also called Calcium release-activated calcium channel protein 1 (CRACM1), is an evolutionary conserved plasma membrane protein. Orai1 is predicted to contain four transmembrane domains with its N- and C-termini in the cell cytoplasm.

ORAI calcium release-activated calcium modulator 1 (Orai1), also known as transmembrane protein 142A (TMEM142A), is encoded by the gene mapped to human chromosome 12q24.31.{49}. The encoded protein contains four transmembrane helices with highly conserved glutamate residues in transmembrane helices 1 and 3.

Immunogen

synthetic peptide located near the C-terminus of human Orai1, conjugated to KLH. The corresponding sequence differs by 2 amino acids in rat and mouse.

Application

Monoclonal Anti-Orai1 antibody has been used in

• immunoblotting,
• immunofluorescence
• immunoprecipitation
• proximity ligation assay.

Monoclonal Anti-Orai1 antibody produced in mouse has been used in proximity ligation assay.

Biochem/physiol Actions

Orai1 essential for store-operated calcium entry in T cells and fibroblasts. Store-operated calcium entry is mediated by Ca²⁺ release-activated Ca²⁺ (CRAC) channels, following Ca²⁺ depletion from the endoplasmic reticulum (ER) stores, caused by stimulation of immune cells. This process is crucial for gene transcription, proliferation and cytokine release. Mutations in two highly conserved glutamate residues, E¹⁰⁶ and E¹⁹⁰, diminish Ca²⁺ influx and promote changes in ion selectivity, providing strong evidence that Orai is a pore subunit of the CRAC channel. Orai1 co-localizes with STIM1 (stromal interaction molecule 1) near the plasma membrane after store depletion. A point mutation in Orai1 is responsible for the genetic defect in store-operated Ca²⁺ entry and I-CRAC in cells of the hereditary severe combined immune deficiency (SCID) syndrome patients.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

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