Anti-Orexin-1 Receptor Antibody (C15-1316-016)

General description

Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Specificity

Recognizes Rat and Human Orexin-1.

Immunogen

A 16 aa peptide near the N-terminus of rat OX1R and a 16 aa peptide near the C-terminus of human OX1R.

Application

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

This Anti-Orexin-1 Receptor Antibody is validated for use in ELISA, WB for the detection of Orexin-1 Receptor.

Western blot: 1-10 μg/mL using Chemiluminescence technique. Protein runs at 45-48 kDa.

ELISA: 1:10,000 -1:100,000 against 50-100 ng/wel immunogen peptide.

Optimal working dilutions must be determined by end user.

Target description

45-48 kDa

Physical form

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

ImmunoAffinity Purified

Storage and Stability

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Hypothalamus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human, mouse. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, western blot: suitable. NCBI accession no.: NM_001525.1. UniProt accession no.: O43613. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... HCRTR1(3061). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.