Anti-Orexin Antibody, Prepro (C15-1316-018)

Specificity

Mouse Prepro-Orexin. Several peptides associated with feeding behavior have been reported recently. Orexins (Orexin-A and Orexin-B) are a family of hypothalamic neuropeptides selectively expressed in the hypothalamus (Sakurai et al. 1998). Orexin-A and Orexin-B are derived from the same precursor (Prepro-orexin) by proteolytic cleavage. Prepro-orexin is an 130 aa peptide with a putative 33 aa secretory sequence, a hydrophobic core followed by residues with small polar side chains. The expression was detected in brain and to a small extent in testis (Sakurai et al. 1998). These neuropeptides bind and activate two closely related Orexin receptors--G protein coupled receptors (GPCRs) OX1R and OX2R.

Immunogen

A 17 mer peptide (designated PPOXA11-A) mapping near the C-terminus of mouse prepro-Orexin.

Application

Anti-Orexin Antibody, Prepro is an antibody against Orexin for use in ELISA, IH & WB.

Research Category
Neuroscience

Research Sub Category
CNS Control of Metabolism

Circadian Rhythm & Sleep

Western blot: 1-10 μg/mL using Chemiluminescence technique

ELISA: 1:10,000 - 100,000

Immunohistochemistry: 2-20 μg/mL.

Optimal working dilutions must be determined by end user.

Physical form

Liquid in PBS with 0.1% BSA.

Storage and Stability

Can be refrigerated at 2-8°C in undiluted aliquots for short term use. Maintain frozen at -20°C in undiluted aliquots for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human, rat. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_001524.1. UniProt accession no.: O43612. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... PPOX(5498). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.