Immunization of mice with chicken egg albumin (ovalbumin/OVA) in a precipitate complex with aluminum hydroxide (alum) is a highly effective means of inducing a potent TH2-mediated immune response. OVA/alum immunized mice produce anti-OVA antibodies predominantly of the IgG1 and IgE isotypes that mediate tissue-specific effector functions in multiple mouse models of chronic inflammation, including allergic asthma, allergic rhinitis, and cutaneous hypersensitivity. When using one of these models, it is often desirable to measure anti-OVA antibody levels in the plasma or serum to determine the effectiveness of the immunization, the activity of a drug, or the effect of a specific gene deletion. The plasma concentration of OVA-specific IgG1, the predominant anti-OVA immunoglobulin isotype found in the serum or plasma of mice immunized with OVA/alum, is typically 1,000-fold greater than that of OVA-specific IgE. Cayman’s Anti-Ovalbumin IgE (mouse) ELISA Kit is an immunometric assay which can be used to measure anti-ovalbumin of the IgE isotype in mouse plasma and serum without prior sample purification. A plate-bound antibody specific for mouse IgE is used to selectively measure ovalbumin-specific IgE, total plasma or serum IgE, which avoids interference from IgG1 binding. A biotin-labeled ovalbumin reagent is then added to quantify the ovalbumin-specific subset of this captured IgE. A monoclonal anti-ovalbumin IgE produced from mice immunized with OVA/alum is used as the standard. The standard curve spans the range of 3.12-100 ng/ml, with an LLOQ of 3.12 ng/ml.
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