Anti-p14ARF (p19ARF) (C15-1316-639)

General description

Tumor suppressor ARF (UniProt Q8N726; also known as Alternative reading frame, ARF, Cyclin-dependent kinase inhibitor 2A, p14ARF, p19ARF) is encoded by the CDKN2A (also known as CDKN2, MLM) gene (Gene ID 1029) in human. p14ARF acts as a tumor suppressor and a key regulator of cellular proliferation that is frequently inactivated in human cancers. It binds to MDM2 and blocks its oncogenic action by inhibiting MDM2-induced degradation of p53. Reported to form a ternary complex with p120(E4F) and p53 that enhances p14ARF-induced G2 cell cycle arrest in a p53-dependent manner. However, it can also induce G2 arrest and apoptosis in a p-53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to E2F1 and MYC and blocks their transcriptional activator activity without affecting MYC transcriptional repression. Human p14ARF is shown to promote the proteasomal degradation of Melanoma antigen-A11 (MAGE-A11) independent of HDM2 E3 ubiquitin ligase or lysine ubiquitination. It inhibits the ability of MAGE-A11 to stabilize and stimulate androgen receptor (AR) or E2F1 transcriptional activity. Ref.: Minges, JT et al. (2015). J. Biol. Chem. 290, 25174-25187. Rizos, H., et al. (2003). J. Biol. Chem. 278, 4981-4989.

Specificity

This rabbit polyclonal antibody targets the C-terminal end sequence present in both ARF spliced isoforms, p14ARF (p19ARF) and smARF, reported by UniProt (Q8N726). This polyclonal antibody does not detect the INK4 spliced isoforms (UniProt P42771) also encoded by the CDKN2A gene.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human p14ARF (p19ARF)/smARF C-terminal end sequence.

Application

Detect tumor suppressor ARF using this rabbit polyclonal Anti-p14ARF (p19ARF), Cat. No. ABC1430, validated for use in Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A representative lot detected p14ARF (p19ARF) immunoreactivity in formalin-fxed, paraffin-embedded human lung tissue sections. An upregulated p14ARF (p19ARF) immunoreactivitiy was found in a lung tissue with squamous cell carcinoma when compared to a normal lung tissue sample (Vonlanthen, S., et al. 1998. Oncogene. 17(21):2779-2785).

Immunoprecipitation Analysis: A representative lot co-immunoprecipitated MDM2 and p53 with p14ARF (p19ARF) either from HeLa cells transfected to overexpress the three proteins or from in vitro transcription/translation reactions (Zhang, Y., et al. 1998. Cell. 92(6):725-234).

Western Blotting Analysis: Representative lots detected HA-tagged human p14ARF (p19ARF) exogenously expressed in HeLa and COS-7 cells, but not in untransfected K562 cells (Vonlanthen, S., et al. 1998. Oncogene. 17(21):2779-2785; Zhang, Y., et al. 1998. Cell. 92(6):725-234).

Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in PC3 cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected p14ARF (p19ARF) in 10 µg of PC3 human prostate cancer cell lysate.

Target description

~17 kDa observed. 13.90 kDa (p14ARF or p19ARF) and 8.731 kDa (smARF) calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_478102. UniProt accession no.: Q8N726. shipped in: ambient. target post-translational modification: unmodified. Gene Information: human ... CDKN2A(1029). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.