ANTI-P16 (AB-1) MOUSE MAB (DCS-50.1/H4)

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-2 mouse myeloma cells. Recognizes the ~16 kDa p16 protein.

Recognizes the ~16 kDa p16 protein in Saos-2 cells and bladder tissue. Sold under license of U.S. Patent 6,482,929. Sold under license of U.S. Patent 5,843,756, 6,090,578, and corresponding patents.

This Anti-p16 (Ab-1) Mouse mAb (DCS-50.1/H4) is validated for use in Immunoblotting for the detection of p16 (Ab-1).

Immunogen

Human

recombinant, human p16

Application

Immunoblotting (0.5-5 µg/ml, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin pH 7.5.

Analysis Note

Negative Control
U2OS cells

Positive Control
Saos-2 cells or bladder tissue

Other Notes

Does not cross-react with the closely related p15 protein. Antibody should be titrated for optimal results in individual systems.

Koh, J., et al. 1995. Nature375, 506.
Lukas, J., et al. 1995. Nature375, 503.
Cheng, J.Q., et al. 1994. Cancer Res.54, 5547.
Jen, J., et al. 1994. Cancer Res.54, 6353.
Kamb, A., et al. 1994 Science264, 436.
Nobori, T., Miura, K., et al. 1994. Nature368, 753.
Okamoto, A., Demetrick, D.J., et al. 1994. Proc. Natl. Acad. Sci. USA91, 11045.
Serrano, M., et al. 1993. Nature366 704.

Legal Information

Sold under license of U.S. Patents 5,843,756, 6,090,578 and corresponding patents.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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