Specificity
P2X3
SPECIES REACTIVITIES:
Rat, mouse and primate. Other species have not been tested.
Immunogen
Synthetic peptide corresponding to the C-terminus of rat P2X3.
Application
Detect P2X3 Receptor using this Anti-P2X3 Receptor Antibody, pain validated for use in IC, IH & WB.
Immunoblotting: 1:500-1:1,000
Immunohistochemistry: 1:500-1:1,000
Immunocytochemistry: 1:500-1:1,000
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5895
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOCYTOCHEMISTRY
P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neuroinflammation & Pain
Physical form
Rabbit serum. Liquid. Contains 0.05% sodium azide.
Storage and Stability
Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, human, primate. manufacturer/tradename: Chemicon®. . technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NM_002559.2. UniProt accession no.: P56373. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... P2RX3(5024). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51271907
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- AB5895
- Temperature Control Device:
- Yes
