ANTI-P300 MOUSE MAB (NM-11)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.

Recognizes the p300 protein in A431, HeLa, and ML-1 cells.

This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

Immunogen

Epitope: within amino acids 2071-2091 near the CBP C-terminus

Human

recombinant, human p300 protein

Application

Frozen Sections (see application references)

Immunoblotting (1 µg/ml)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 0.05 M sodium phosphate buffer, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
A431, HeLa, or ML-1 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.

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