General description

Cell division protein kinase 5 (Cdk5) is a serine/threonine kinase and shares homology to other Cdks. It belongs to cdk family.

Immunogen

synthetic peptide corresponding to the C-terminus of p35 (Cdk5R), (human, amino acids 192-209).

Application

Anti-p35 (Cdk5 Regulator) antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Cell division protein kinase 5 (Cdk5) kinase activity can be detected mainly in postmitotic neurons in the central nervous system (CNS). Cdk5/p35 activity is required in the mature CNS. Cdk5 phosphorylates dopamine and cyclic adenosine 3′,5′-monophosphate-regulated phosphoprotein of 32 kDa (DARPP-32) and Syntaxin-binding protein 1 (Munc18), which in turn affects synaptic vesicle endocytosis. Deregulation of Cdk5 activity contributes to the pathogenesis of neurodegenerative diseases such as Alzheimer′s disease (AD). It mediates neuronal migration during cortical development. Cdk5-/- mice exhibit embryonic lethality associated with disruption of the cortical laminar structures in the cerebral cortex, olfactory bulb, hippocampus, and cerebellar cortex. Mice lacking p35 display defects in cortical lamination and fasciculation of axonal fibers. In developing mice brain, Cdk5/p35 interacts with Reelin/Dab1 and positions of cortical neurons.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

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