ANTI-P38/SAPK2 CLONE 2F11

General description

Mitogen-activated protein kinase 14 (EC 2.7.11.24; UniProt P47811; also known as CRK1, MAP kinase 14, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, p38a, p38alpha, p38MAPK, SAPK2a, Stress-activated protein kinase 2a) is encoded by the Mapk14 (also known as Crk1, Csbp1, Csbp2, Mxi2) gene (Gene ID 26416) in murine species. p38 mitogen-activated protein kinases (MAPKs) constitute a group of stress-activated serine/threonine-specific kinases (SAPKs), including p38α (MAPK14/SAPK2A), p38β (MAPK11/SAPK2B), p38γ (ERK6/MAPK12/SAPK3), and p38δ (MAPK13/SAPK4), that mediate cellular signal transduction and play a vital role in numerous biological processes. p38α and p38β are ubiquitously expressed, while p38γ and p38δ are differentially expressed depending on tissue type. Sequence comparisons show approximately 60% identity within the p38 group. but only 40-45% to the other three MAP kinase family groups, extracellular signal-regulated kinases (ERKs), c-jun N-terminal or stress-activated protein kinases (JNK/SAPK), and ERK/big MAP kinase 1 (BMK1). All four p38 kinases contain a conserved Thr-Gly-Tyr (TGY) dual phosphorylation motif and are activated by dual kinases called MAP kinase kinases (MKKs).

Specificity

Clone 2F11 recognizes both p38alpha/SAPK2a and p38beta/SAPK2b.

Immunogen

Full-length murine p38/SAPK2a GST fusion protein.

Application

Research Category
Signaling

Research Sub Category
MAP Kinases

This Anti-p38 Antibody, clone 2F11 is validated for use in Western Blotting for the detection of SAPK2.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected p38/SAPK2 in 10 µg of mouse (C2C12, NIH/3T3, RAW264), rat (L6, PC12), and human (HEK293, HeLa, HepG2, HUVEC, Jurkat) cell lysates, and in 10 µg of mouse brain homogenate.
Western Blotting Analysis: Representative lots detected p38 in human A549 epidermoid carcinoma cell and WI38-VA13 fibroblast lysates (Song, J.Y., et al. (2010). J. Biol. Chem. 285(12):9067-9076; Song, J.Y., et al. (2009). Cancer Lett. 283(2):135-142).
Western Blotting Analysis: A representative lot detected p38 in mouse embryonic stem cell lysate (Wu, X., et al. (2007). Dev. Dyn. 236(10):2767-2778).

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected p38/SAPK2 in 10 µg of A431 cell lysate.

Target description

~38 kDa observed.

Linkage

Replaces: 05-454

Physical form

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.