Anti-p47-phox Antibody (C15-1317-385)

General description

Neutrophil cytosol factor 1 (UniProt Q09014), also known as 47 kDa neutrophil oxidase factor, NADPH oxidase subunit, Ncf-1, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, p47phox) is encoded by the Ncf1 gene in murine species. Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of mouse p47-phox.

Application

Anti-p47-phox Antibody is an antibody against p47-phox for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p47-phox in mouse cerebral cortex and mouse small intestine.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected p47-phox in 10 µg of Raji cell lysate.

Target description

~47 kDa observed

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. species reactivity (predicted by homology): rat (based on 100% sequence homology). technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_001272966. UniProt accession no.: Q09014. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Ncf1(17969). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.