Anti-PAI-1 (Ab-1) Mouse mAb (2)

General description

Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.

Recognizes both active and latent forms of the ~50 kDa PAI-1 protein in conditioned media from dexamethasone-treated HT-1080 cells. Sold under license of U.S. Patent 5,422,245.

This Anti-PAI-1 (Ab-1) Mouse mAb (2) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of PAI-1 (Ab-1).

Immunogen

Human

partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see application references)

Immunocytochemistry (see application references

Neutralization Studies (see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Analysis Note

Positive Control
Conditioned media from dexamethasone-treated (1 µM) HT-1080 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.

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Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.
Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.
Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.

Legal Information

Sold under license of U.S. Patent 5,422,245.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany