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ANTI-PAI-1 (AB-1) MOUSE MAB 1PC X 100UG

Sigma Aldrich

Catalog No.
C15-1468-640
Manufacturer No.
IM29L-100UG
Manufacturer Name
Sigma-Aldrich
Quantity
100
Unit of Measure
UG
Price: $875.68
List Price: $972.97

Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.

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General description

Purified mouse monoclonal antibody (see application references). Recognizes both active and latent forms of the ~50 kDa PAI-1 protein.

Recognizes both active and latent forms of the ~50 kDa PAI-1 protein in conditioned media from dexamethasone-treated HT-1080 cells. Sold under license of U.S. Patent 5,422,245.

This Anti-PAI-1 (Ab-1) Mouse mAb (2) is validated for use in Immunoblotting, Immunoprecipitation, Immunocytochemistry for the detection of PAI-1 (Ab-1).

Immunogen

Human

partially purified PAI-1 from conditioned medium of dexamethasone-treated HT1080 human cells

Application

Immunoblotting (1 µg/ml)

Immunoprecipitation (see application references)

Immunocytochemistry (see application references

Neutralization Studies (see application references)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from a volatile buffer, 100 µg BSA.

Reconstitution

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h.

Analysis Note

Positive Control
Conditioned media from dexamethasone-treated (1 µM) HT-1080 cells

Other Notes

Antibody should be titrated for optimal results in individual systems.

Janicke, F., et al. 1994. Cancer Research.54, 2527.
Lawrence, D. A., et al. 1994. J. Biol. Chem.269, 15223.
Nagayama, M., et al. 1994. Cancer73, 1398.
Petersen, H., et al. 1994. Cancer Res.54, 120.
Keijer, J., et al. 1991. Blood78, 401.
Blasi, F. and Verde, P., 1990. in Seminars in Cancer Biol.Vol. 1, 153.
Declerck, P. J. and Collen, D., 1990. Thrombosis Res.Supplement X 3.
Nielsen, L. S., et al. 1986. Thrombosis and Haemostasis.55, 206.

Legal Information

Sold under license of U.S. Patent 5,422,245.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

UPC:
51202414
Condition:
New
Weight:
1.00 Ounces
HazmatClass:
No
WeightUOM:
LB
MPN:
IM29L-100UG

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