ANTI-PANNEXIN-1/PANX1 (C005B-241326)

General description

Pannexin-1 (UniProt Q96RD7; also known as Innexin) is encoded by the PANX1 (also known as MRS1, PX1) gene (ORF UNQ2529/PRO6028; Gene ID 24145) in human. Pannexin 1 is a high-conductance, voltage-gated channel protein widely expressed in many organs and tissues, including the sensory systems (eye, inner ear, taste buds, and the olfactory epithelium) and both neuronal and non-neuronal cell types. Three pannexin family genes (PANX1-3) have been identified in human and mouse genomes. Pannexin proteins share structural and functional similarities and play important roles in many physiological and pathological processes, such as ATP release, Ca2+ homeostasis, synaptic neurotransmitters release, apoptosis, and immunological response. Pannexin-1 deficiency causes caspase-3 activation and apoptosis induction of hair cells in cochlear spiral limbus (SLM), resulting in hearing loss among Panx1-/- mice. Panx1 is also expressed in the retina, lens and cornea of the eye, where it plays an important role in mediating the crosstalk between immune and neuronal cells. Pannexin-1 channel opening, frequently together with the activation of purinergic receptors by extracellular ATP, is reported to have a great implication in diverse pathological conditions, including ischemia, trauma, HIV infection, inflammation, migraine, and cancer cell growth.

Specificity

Expected to react with both spliced isoforms reported by UniProt (Q96RD7-1 & Q96RD7-2).

Immunogen

Epitope: C-terminal

GST-tagged recombinant human Pannexin-1/PANX1 C-terminal fragment.

Application

Anti-Pannexin-1/PANX1 Antibody is an antibody against Pannexin-1/PANX1 for use in Western Blotting, Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Pannexin-1/PANX1 in human testis and mouse kidney tissue sections.

Research Category
Neuroscience

Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in human cortex tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected Pannexin-1/PANX1 in 10 µg of human cortex tissue lysate.

Target description

~55 kDa observed. 55-58 kDa (membrane glycosylated) and 43 kDa (cytosolic non-glycosylated) expected. 48.1 kDa (isoform 1) & 47.6 kDa (isoform 2) calculated.

Linkage

Replaces: AB9886

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse, rat. species reactivity (predicted by homology): gorilla (based on 100% sequence homology), chimpanzee (based on 100% sequence homology). technique(s): immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_056183. UniProt accession no.: Q96RD7. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... PANX1(24145). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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