General description
Protein A purified mouse monoclonal antibody. Recognizes poly-ADP-ribose.
Recognizes poly(ADP)-ribose (PAR) in etopside-treated HL-60 cells.
This Anti-PAR (Ab-1) Mouse mAb (10H) is validated for use in Immunoblotting, Immunocytochemistry for the detection of PAR (Ab-1).
Immunogen
Bovine
purified poly(ADP-ribose)
Application
Immunoblotting (2.5 µg/ml)
Immunocytochemistry (5-20 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Physical form
In 100 mM NaCl, 50 mM HEPES, 1% BSA, pH 7.4.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
Normal mouse IgG
Positive Control
Etoposide-treated HL-60 cells
Other Notes
Kawamitsu, H., et al. 1984. Biochemistry23, 3771.
PAR can be induced in HL-60 cells by treatment with 67 µM etoposide for 3.5 h. Antibody should be titrated for optimal results in an individual system.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: 10H, monoclonal. Assay: ≥. 95% (SDS-PAGE). form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: human, Drosophila, rat, mouse. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG3. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51202401
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AM80-100UG