ANTI-PAX1 CLONE 5A2 (C15-1887-913)

General description

Paired box protein Pax-1 (UniProt: P09084) is encoded by the Pax1 (also known as Pax-1) gene (Gene ID: 18503) in murine species. Pax-1 is a transcriptional activator that plays a role in the formation of segmented structures of the embryo and has important role in the normal development of the vertebral column. Mutations in Pax1 gene are associated with vertebral malformations. Undulated homozygous mice exhibits vertebral malformations along the entire rostro-caudal axis. Pax genes are vital for the specification of progenitor cells and for maintenance of progenitor cell fate, including that of cancer cells. Pax1 gene is silenced by methylation in ovarian and cervical cancers and may serve as a tumor suppressor gene. The methylation levels of Pax1 gene is significantly higher in tumor tissues compared to non-tumor paracancerous tissues. Hence, methylation status of Pax1 may serve as useful biomarkers for the detection of cervical cancer and is also a good prognostic indicator for oral squamous cell carcinoma. (Ref.: Huang J et al (2017). Int. J. Environ. Res. Public Health. 14(2). pii: E216).

Specificity

Clone 5A2 detects Paired box protein Pax-1 in murine tissues.

Immunogen

GST-tagged full-length murine recombinant paired box protein Pax-1.

Application

Detect Paired box protein Pax-1 using this rat monoclonal Anti-Pax1, clone 5A2, Cat. No. MABE1115-25UG, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Research Category
Apoptosis & Cancer

Western Blotting Analysis: A representative lot detected Pax1 in Western Blotting applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]).

Immunohistochemistry Analysis: A representative lot detected Pax1 in Immunohistochemistry applications (Feederle, R., et. al. (2016). Monoclon Antib Immunodiagn Immunother. [Epub ahead of print]; Kist, R., et. al. (2014). PLoS Genet. 10(10):e1004709).

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Pax1 in Embryonic mouse head (E14.5) (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

Western Blotting Analysis: A 1:10 dilution from a representative lot detected Pax1 in d11.5 embryonic vertebral column tissue lysates (Courtesy of Dr. Heiko Peters, Ph.D., Institute of Genetic Medicine, Newcastle University, United Kingdom).

Quality

Evaluated by Western Blotting in mouse esophagus tissue lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Pax1 in 10 µg of m ouse esophagus tissue lysate.

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.