Anti-PDE4D Antibody (C15-1317-827)

General description

Phosphodiesterase 4D (PDE4D) is a member of the PDE cyclic nucleotides. PDE4D is known to be regulated by protein kinase C (PKC)-Raf-MEK-ERK and also by cAMP in vascular smooth muscle cells. PDE4D is essential to have equilibrium in relaxing and contracting cues to airway smooth muscle. Down-regulation of PDE4D may be associated with analgesic and anti-inflammatory effects. It has also been linked to the risk of stroke, specifically common polygenic stroke.

Specificity

This antibody recognizes PDE4D at the C-terminus.

Immunogen

GST-tagged recombinant protein corresponding to the C-terminus of human PDE4D.

Application

Anti-PDE4D Antibody is an antibody against PDE4D for use in WB.

Quality

Evaluated by Western Blot in rat brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PDE4D on 10 µg of rat brain tissue lysate.

Target description

~ 59, 68, 85, 90, and 105 kDa observed. UniProtKB/Swiss-Prot Q08499 (PDE4D_HUMAN) entry describes 12 isoforms produced by alternative splicing: Isoform 1 at 91.115 kDa, Isoform 2 at 76.369 kDa, Isoform 3 at 68.607 kDa, Isoform 4 at 66.376 kDa, Isoform 5 at 57.792 kDa, Isoform 6 at 84.428 kDa, Isoform 7 at 23.839 kDa, Isoform 8 at 59.113 kDa, Isoform 9 at 77.705 kDa, Isoform 10 at 76.816 kDa, Isoform 11 at 84.662 kDa, and Isoform 12 at 24.429 kDa

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. clone: polyclonal. species reactivity: rat, human. species reactivity (predicted by homology): chimpanzee (based on 100% sequence homology), mouse (based on 90% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_001098101. UniProt accession no.: Q08499. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... PDE4D(5144). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.