Anti-PECAM-1 Antibody, clone 390, FITC conjugated

General description

PECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.

Specificity

CBL1337 recognizes mouse PECAM-1, a 130-140 kDa type 1 integral membrane glycoprotein. This antibody has been shown to inhibit the aggregation of L cells transfected with a variant form of PECAM-1 (DeLisser, 1993).

Immunogen

Mouse leukocyte cell line 32D

Application

Flow Cytometry: = 1 μg per 10e6 cells in 100 μL volume

Immunohistochemistry: acteone fixed frozen tissue sections.

Optimal working dilutions must be determined by the end user.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

This Anti-PECAM-1 Antibody, clone 390, FITC conjugated is validated for use in FC, IH for the detection of PECAM-1.

Physical form

Purified immunoglobulin conjugated to fluorescein. Liquid in in phosphate buffered saline containing 0.1% sodium azide.

Storage and Stability

Maintain at 2°-8°C for up to 12 months from date of receipt. DO NOT FREEZE.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.