ANTI-PERICENTRIN CLONE 2H11 ZOOMAB RABBIT MONOCLONAL (C15-1670-773)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 2H11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects human Pericentrin. It targets an epitope within 132 amino acids from the N-terminal region.

Immunogen

GST/His-tagged recombinant fragment corresponding to 132 amino acids from the N-terminal region of human Pericentrin.

Application

Anti-Pericentrin, clone 2H11 ZooMAb, Cat. No. ZRB1318, a Rabbit monoclonal antibody that targets human Pericentrin and is tested in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry, and Western Blotting.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Pericentrin in HeLa, A431, HUVEC and HepG2 cell lines.

Affinity Binding Assay: A representative lot of this antibody bound recombinant Pericentrin with a KD of 2.7 x 10-9 in an affinity binding assay.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Pericentrin in HeLa and HEK293 cell lysates.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Pericentrin (UniProt: O95613; also known as Kendrin, Pericentrin-B) is encoded by the PCNT (also known as KIAA0402, PCNT2) gene (Gene ID: 5116) in human. Pericentrin is a highly conserved protein of the pericentriolar material and is an integral component of the filamentous matrix of the centrosome involved in the initial establishment of organized microtubule arrays in both mitosis and meiosis. The centrosomal localization of pericentrin is shown to be mediated by a pericentrosomal matrix targeting motif, known as PACT domain, in its C-terminal region. Pericentrin plays a role, together with DISC1, in the microtubule network formation. It is also reported to prevent premature centrosome splitting during interphase by inhibiting NEK2 kinase activity at the centrosome. It is also involved in retinal development by regulating photoreceptor ciliogenesis and photoreceptive outer segment formation and plays a role in ciliary development in olfactory receptor neurons. Pericentrin is shown to bind to calmodulin by a C-terminal binding site (aa 3195-3208) and is essential for its functions at centrosomes. During mitosis, pericentrin is cleaved to remove CDK5RAP2 that promotes centriole disengagement and subsequent centriole separation. Mutations in PCNT2 gene are known to cause microcephalic osteodysplastic primordial dwarfism that is characterized by microcephaly and short stature. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Falk, N., et al. (2018). J. Cell Sci. 131(16); jcs218487).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

30 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.