RABBIT ANTI-HORSERADISH PEROXIDASE

General description

Antiserum is determined to be immunospecific for horseradish peroxidase and does not inhibit the enzymatic activity of the peroxidase.

Peroxidase is a hemoprotein.

The fractionation procedure yields primarily the immunoglobulin fraction of antiserum. If necessary, the fractionated antiserum is adsorbed using solid phase techniques. Antiserum is determined to be immunospecific by immunoelectrophoresis (IEP), against purified and crude horseradish peroxidase
The gel is stained with DAB (diaminobenzidine) to ensure that the antibody does not inhibit the enzymatic activity of the peroxidase. Identity and purity of the antibody is established by immunoelectrophoresis. Electrophoresis of the antibody preparation followed by diffusion against anti-rabbit IgG and anti-rabbit whole serum results in single arcs of precipitation in the γ region
Each mL will precipitate a minimum of 0.25 mg of peroxidase at equivalence. May be used to stain axons and synaptic terminals in Drosophila embryo fillets.

Immunogen

Purified peroxidase from horseradish

Application

Anti-Peroxidase antibody produced in rabbit has been used in:

• immunoblot assays
• enzyme-linked-immunosorbent assay (ELISA)
• immunoprecipitation

Biochem/physiol Actions

Peroxidase helps in catalyzing the oxidation of a number of substrates with the help of hydrogen peroxide.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2

Reconstitution

Reconstitute with deionized water.

Analysis Note

Specificity is determined by immunoelectrophoresis using a crude peroxidase extract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.