Anti-PGAM5 Antibody (C15-1316-744)

General description

PGAM5 is a mitochondrial specific phosphatase specific for serine/threonine residues. PGAM5 principally dephosphorylates and activates MAP3K5 kinase a critical player in many cancers. PGAM5 also acts as a central mediator for programmed necrosis or necroptosis, induced by TNF, ROS or calcium. In the mitochondria, PGAM5 may also function as a regulator of mitochondrial dynamics and reconstruction. Also PGAM5 functions in the cellular stress response and regulates the removal of damaged mitochondria via mitophagy. PGAM5 itself is regulated at the protein level by the mitochondrial specific rhomboid protease PARL, which cleaves the N-terminal of PGAM5 in response to mitochondrial depolarization.

Specificity

This antibody detects the -L(long) isoform and -S(short) isoform of PGAM5.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human PGAM5 near the N-terminal.

Application

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-PGAM5 Antibody is validated for use in Western Blotting for the detection of PGAM5.

Quality

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected PGAM5 in 10 µg of HT-29 cell lysate.

Target description

~32/34 kDa observed.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): primate (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rodent (based on 100% sequence homology), monkey (based on 100% sequence homology), hamster (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_001164014. UniProt accession no.: Q96HS1. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... PGAM5(192111). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.