ANTI-PHD FINGER PROTEIN 14 (PHF14) (C005B-240720)

General description

PHD finger protein 14 (UniProt: O94880) is encoded by the PHF14 (also known as KIAA0783) gene (Gene ID: 9678) in human. PHF14 is a member of the PHD finger protein family that serves as a chromatin-binding protein. It contains four putative PHD fingers, two coiled-coil regions, and four zinc fingers. PHF14 is highly conserved from C. elegans to humans and is considered as a transcriptional regulator of target gene expression. PHF14 has been identified as a regulator of mesenchyme growth in embryonic tissues. Two isoforms of PHF14 have been described that are produced by alternative splicing. PHF14 interacts with histones via its PHD1 and PHD3 domains indicating its role in epigenetic regulation. It is involved in chromatin-mediated transcriptional regulation and chromatin dynamics. During cell division, PHF14 is shown to be directly bound to and co-localized with KIF4A to form a functional complex. PHF14 is shown to be overexpressed in lung cancer and this overexpression is associated with poor prognosis of non-small cell lung carcinoma. (Ref.: Zhang, L., et al. (2017). Oncotarget. 8(12); 19684-19698; Yang, B., et al. (2017). Sci Rep. 7; 39888).

Specificity

This rabbit polyclonal antibody detects PHD Finger Protein 14 in human cells. It targets an epitope within 16 amino acids from the N-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal half of human PHD finger protein 14.

Application

Anti-PHD finger protein 14 (PHF14) Antibody, Cat. No. ABE1359, is a highly specific rabbit polyclonal antibody that detects PHD finger protein 14 and has been tested for use in Immunocytochemistry and in Western Blotting.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected PHD finger protein 14 (PHF14) in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PHD finger protein 14 (PHF14) in HEK293 cell lysate.

Target description

100.0 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (based on 100% sequence homology). packaging: antibody small pack of 25 . μ. L. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_055475.2. UniProt accession no.: O94880. target post-translational modification: unmodified. Gene Information: human ... PHF14(9678). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.