Anti-phospho Angiomotin Antibody (Ser175) (C15-1317-687)

General description

Angiomotin is a cell surface protein that plays a central role in tight junction maintenance and the uptake of proteins at tight junctions but also appears to regulate endothelial cell response and their migration toward growth factors as well as their response to angiostatin. Thus Angiomotin plays a role in blood vessel formation, cell migration, protein localization, chemotaxis, cellular polarity and many more cell-movement/migration activities. Angiomotin is a component of a multi-protein complex associated at tight junctions that includes ARHGAP17, AMOT, MPP5/PALS1, INADL/PATJ and PARD3/PAR3 proteins. Angiomotin is expressed in endothelial cells and found in placenta, blood vessels and skeletal muscle tissue, as well as at the leading edge of lamellipodia and cellular processes.

Specificity

This antibody only detects p130 isoform.

Immunogen

Epitope: Phosphorylated Ser175

Linear peptide corresponding to human phospho Angiomotin (Ser175).

Application

Anti-phospho Angiomotin Antibody (Ser175) | ABS1045 is an antibody against phospho Angiomotin Antibody for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Quality

Evaluated by Western Blotting in HEK293 cell lysate transfected with LATS (Angiomotin Kinase).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho Angiomotin (Ser175) in 10 µg of HEK293 transfected with LATS.

Target description

~150 kDa observed. The calculated molecular weight is 118 kDa, however phospho Angiomotin (Ser175) has been shown as a ~150 kDa band in western blots (Yi, C., et al. (2011). Cancer Cell. 19(4):527-540).

Physical form

Format: Purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

T-gel

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. species reactivity (predicted by homology): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology). technique(s): western blot: suitable. NCBI accession no.: NP_001106962. UniProt accession no.: Q4VCS5. shipped in: wet ice. target post-translational modification: phosphorylation (pSer175). Gene Information: human ... AMOT(154796). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.