Anti-phospho-BRCA2 (Ser3291) Antibody (C15-1316-610)

General description

BRCA2 (Breast Cancer Type 2 susceptibility protein) is a tumor suppressor protein encoded by the BRCA2 gene.
The protein encoded by this gene plays a direct role in error-free double-strand break repair and homologous recombination. BRCA2 shares similarities with the tumor suppressor gene BRCA1. Inherited mutations in BRCA2 as well as BRCA1 are associated with an increased risk of developing breast or ovarian cancer. Although the structures of the BRCA1 and BRCA2 genes are very different, some functions are similar. The proteins made by both genes are essential for repairing damaged DNA and interact with the protein produced by the RAD51 gene. Together these three proteins play a role in maintaining the stability of the human genome and prevent dangerous gene rearrangements that can lead to the development of cancer.

Specificity

This antibody recognizes human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.

Immunogen

Epitope: Internal Domain surrounding Ser3291

KLH-conjugated linear peptide corresponding to human BRCA2 phosphorylated at and around the internal domain surrounding Ser3291.

Application

Anti-phospho-BRCA2 (Ser3291) Antibody is a rabbit polyclonal antibody for detection of phospho-BRCA2 (Ser3291), also known as BRCA1/BRCA2-containing complex subunit 2 & Fanconi anemia group D1 protein 3, has been validated in ICC, IHC, DB.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected BRCA2 in MCF-7 cells.

Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected BRCA2 in metastatic ductal carcinoma tissue.

Research Sub Category
Cell Cycle, DNA Replication & Repair

Oncoproteins

Quality

Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.

Dot Blot Analysis: 2 µg/mL of this antibody detected BRCA2 in modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2.

Target description

~ 390 kDa

Analysis Note

Control
Modified and non modified peptides of phosphorylated and non-phosphorlyated BRCA2

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rhesus macaque, horse, human. species reactivity (predicted by homology): rat (based on 100% sequence homology), mouse (based on 100% sequence homology), hamster (based on 100% sequence homology), cat (based on 100% sequence homology), equine (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology). technique(s): dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable. NCBI accession no.: NP_000050.2. UniProt accession no.: P51587. shipped in: wet ice. target post-translational modification: phosphorylation (pSer3291). Gene Information: human ... BRCA2(675). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.