ANTI-PHOSPHO-CYTOKERATIN-8 (K8) (SER73) CLONE LJ4 (C005B-448446)

General description

Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8 Keratin-8, K8, Type-II keratin Kb80) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratin-8 is a type II, neutral to basic, protein of the intermediate filament family that together with Cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. Cytokeratin-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is shown to be highly conserved in all type II keratins. Phosphorylation at Serine 73 is reported to increase during cellular stress, including hear and drug exposure. However, under normal conditions serine 73 remains largely dephosphorylated. Cytokeratin-8 can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. Mutations in KRT8 gene have been linked to liver cirrhosis that is characterized by severe panlobular liver-cell swelling with Mallory body formation, prominent pericellular fibrosis, and marked deposits of copper. Two isoforms of Cytokeratin-8 have been described that are produced by alternative splicing.

Specificity

Clone LJ4 detects human cytokeratin-8 phosphorylated on serine 73 and murine cytokeratin-8 phosphorylated on serine 79.

Immunogen

Cytokeratin-8 purified from HT29 cells treated with okadaic acid (1 mg/ml, 2h).

Application

Anti-phospho-cytokeratin-8 (K8) (Ser73), clone LJ4, Cat. No. MABT344, is a mouse monoclonal antibody that detects human type II cytoskeletal 8 phophrorylated on serine 73 and murine cytokeratin-8 phophorylated on serine 79 and has been tested for use in ELISA, Immunocytochemistry, Immunofluoresce

Immunofluorescence Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunofluorescence applications (McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).

Immunoprecipitation Analysis: A representative lot immunoprecipitaated phospho-cytokeratin-8 (K8) (Ser73) in Immunoprecipitation applications (Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).

Immunocytochemistry Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Immunocytochemistry applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70).

Western Blotting Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in Western Blotting applications (Toivola, D.M., et. al. (2002). Mol Biol Cell. 13(6):1857-70; Ku, N.O., et. al. (2003). Hepatology. 37(5):1006-14; Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73; McIntosh, P.B., et. al. (2010). J Cell Sci. 123(Pt 16):2810-22).

ELISA Analysis: A representative lot detected phospho-cytokeratin-8 (K8) (Ser73) in ELISA applications (Liao, J., et. al. (1997). J Biol Chem. 272(28):17565-73).

Quality

Evaluated by Western Blotting in lysates from HeLa cells treated with Calyculin A/Okadaic Acid.

Western Blotting Analysis: 2 µg/mL of this antibody detected phospho-cytokeratin-8 (K8) (Ser73) in lysate from HeLa cells treated with Calyculin A/Okadaic Acid, but not in lysate from untreated cells.

Target description

~53 kDa observed; 53.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.