ANTI-PHOSPHO HISTONE H3 (SER10) ANTIBODY ALEXA FLUOR« 488 CONJUGATE

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

Linear peptide corresponding to phospho-Histone H3 (Ser10).

Application

Anti-phospho-H3 (Ser10), Alexa Fluor^™ 488 Conjugate is a purified rabbit polyclonal that detects Histone H3 phosphorylated at serine 10. This Ab is labeled with Alexa Fluor 488 and validated in IC. The unconjugated version of this antibody is highly published and proven in ICC, IP & WB.

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected phospho Histone H3 (Ser10) in NIH/3T3 cells.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Histone H3 (Ser10) in HeLa cell lysate.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.

Alexa Fluor^™ is a registered trademark of Life Technologies.

Target description

~17 kDa observed

Physical form

Protein A purified

Purified rabbit conjugate in buffer containing PBS, 15 mg/ml BSA, and 0.1% Sodium Azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.