Anti-phospho Histone H3 (Ser57) Antibody, Trial Size (C15-1317-029)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Immunogen

KLH-conjugated linear peptide corresponding to human Histone H3 phosphorylated at Ser57.

Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho Histone H3 (Ser57) in HeLa cells.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser57) from 500 µg of colcemid treated HeLa acid extract.

Dot Blot (Specificity) Analysis: 0.005 µg/mL from a representative lot detected phospho Histone H3 (Ser57) in a panel containing various modified and non-modified Histone H3 peptides.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-phospho Histone H3 (Ser57) Antibody, Trial Size is validated for use in western blotting, ICC, IP & dot blot for the detection of phospho Histone H3 (Ser57).

Quality

Evaluated by Western Blotting in colcemid treated HeLa acid extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Histone H3 (Ser57) in 10 µg of colcemid treated HeLa acid extract.

Target description

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, mouse. technique(s): dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_003520. UniProt accession no.: P68431. shipped in: wet ice. target post-translational modification: phosphorylation (pSer57). Gene Information: human ... HIST1H3F(8968). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.