Anti-phospho HSP27 (pS82) Antibody (C15-1316-094)

General description

Heat Shock Protein 27 (HSP27) is a 27 kDa member of a family of proteins whose expression and function are stimulated by heat shock and other stress stimuli. A major function of these proteins is to serve as chaperones that bind to and stabilize the active conformation of other proteins. HSP27, along with other members of the small HSP group, possesses a C-terminal α-crystalline homology domain. HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Cytoplasmic HSP27 exists in multiple complexes. One complex consists of HSP27, Akt (PKB), MAPKAP-kinase 2, and p38 MAPK. The presence of HSP27 in this complex is required for Akt activation by stress stimuli. Another complex consists of HSP27 and the IKK complex. HSP27 is also an actin capping protein that binds to the barbed (growing) ends of actin filaments, thereby inhibiting filament extension. Phosphorylation of HSP27 on serine 82 by MAPKAP-kinase 2 leads to HSP27 dissociation from the Akt/MAPKAP-kinase 2/p38 complex and from actin filaments, and stimulates HSP27 binding to the IKK complex.

Specificity

Human HSP27. This antibody does not detect endogenous mouse HSP25 [pS86] protein in extracts of NIH3T3 cells treated with anisomycin; however, interaction with the human HSP27 protein is inhibited by the phosphopeptide used to generate the mouse HSP25 [pS86] antibody.

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human HSP27 that contains serine 82.

Application

Anti-phospho HSP27 (pS82) Antibody detects level of phospho HSP27 (pS82) & has been published & validated for use in WB.

Research Category
Protein Trafficking

Research Sub Category
Chaperones

Linkage

Replaces: 04-448

Physical form

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide.

Format: Purified

Storage and Stability

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Analysis Note

Control
HeLa cells treated with TNF-α.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_001540.2. UniProt accession no.: P04792. shipped in: dry ice. target post-translational modification: phosphorylation (pSer82). Gene Information: human ... HSPB1(3315). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2.