Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody (C15-1317-126)

General description

There are two kinds of IGF receptors based on their relative affinities for IGF-1 versus IGF-2. The IGF-1 receptor (IGF-1R) prefers IGF-1 over IGF-2 and weakly binds insulin. IGF-1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kDa) and two beta (95 kDa) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF-1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF-1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by mediating signaling pathways that enhance cell survival.

Specificity

This antibody recognizes IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166. Please note that some sources list the phsophorylation sites as Tyr1131/Tyr1135/Tyr1136. The difference in the amino acid sequence is due to a 30 amino acid signal chain that is removed due to protein processing.

Immunogen

Epitope: Phosphorylated Tyr1161/Tyr1165/Tyr1166.

KLH-conjugated linear peptide corresponding to human IGF-1R phosphorylated at Tyr1161/Tyr1165/Tyr1166.

Application

Anti-phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) Antibody is an antibody against phospho-IGF-1R (Tyr1161/Tyr1165/Tyr1166) for use in WB, Cell Function Assay.

Research Category
Epigenetics & Nuclear Function

Signaling

Research Sub Category
Insulin/Energy Signaling

Insulin/Energy Signaling

Western Blot Peptide Inhibition Analysis: A representative lot detected IGF-1R in IGF1 treated HEK293 cell lysate.

Quality

Evaluated by Western Blot in IGF1treated HEK293 cell lysate.

Western Blot Analysis: A 1:200 dilution of this antibody detected IGF-1R in IGF1 treated HEK293 cell lysate.

Target description

~95 kDa observed. IGF-1R can be cleaved into an Insulin-like growth factor 1 receptor alpha (aa 31-736) and beta (aa 741-1367) chain. Both chains are known to contain multiple potential glycosylation sites. Distinct beta-subunits are present in hybrid insulin-like-growth-factor-1 receptors in the central nervous system. (Moss, A.M., et al. (1993). Biochem J. 294( Pt 3):685-692.)

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
IGF1 untreated and treated HEK293 cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, pig. species reactivity (predicted by homology): mouse (based on 100% sequence homology), Xenopus (based on 100% sequence homology), porcine (based on 100% sequence homology), chicken (based on 100% sequence homology), zebrafish (based on 100% sequence homology), rat (based on 100% sequence homology). technique(s): cell based assay: suitable, western blot: suitable. NCBI accession no.: NP_000866. UniProt accession no.: P08069. shipped in: wet ice. target post-translational modification: phosphorylation (pTyr1161/pTyr1165/pTyr1166). Gene Information: human ... IGF1R(3480). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.