ANTI-PHOSPHO-ITCH (PT222) ANTIBODY (C005B-239472)

General description

ITCH is an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. ITCH regulates the transcriptional activity of several transcription factors including the hematopoietic transcription factor NF-E2, and may play an important role in the regulation of immune response. ITCH down-regulates Epstein-Barr virus LMP2A activity in B cell signaling, and also interacts with NOTCH1, OCLN, JUN, and JUNB. ITCH has a wide tissue distribution and is localized within the nucleus and cytoplasm. Two ITCH splice variants have been described; an approximately 103 kDa form as well as a truncated form (~98 kDa) lacking 42 amino acids in the N-terminal half of the protein. ITCH contains a MAP Kinase binding site and its activity is modulated by phosphorylation at multiple threonine residues by JNK1.

Specificity

Human ITCH phosphorylated on threonine 222 (T222).

Immunogen

Synthetic peptide corresponding to a portion of human ITCH encompassing the phosphorylated Threonine at position 222 (PPPpTPRRP).

Application

Research Category
Protein Trafficking

Research Sub Category
Ubiquitin & Ubiquitin Metabolism

This Anti-phospho-ITCH (pT222) Antibody is validated for use in DB, WB for the detection of phospho-ITCH (pT222).

Western Blot: Recommended Dilution 1 - 5 μg/mL
Dot Blot

Physical form

Affinity purified immunoglobulin. Liquid in Tris-citrate/phosphate buffer, pH 7-8, containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C for up to 12 months from date of receipt

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): dot blot: suitable, western blot: suitable. NCBI accession no.: NM_031483.3. UniProt accession no.: Q96J02. shipped in: wet ice. target post-translational modification: phosphorylation (pThr222). Gene Information: human ... ITCH(83737). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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