General description
JAK-2 (Janus Kinase 2) belongs to the emerging family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK-2. JAK-2 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to CIS elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system by stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies. Within the JAK-2 kinase domain, there is a region that has considerable sequence homology to the regulatory region of the insulin receptor.
Specificity
This antibody recognizes JAK-2 when phosphorylated on Tyr570.
Immunogen
Epitope: Phosphorylated Tyr570
KLH-conjugated linear peptide corresponding to human JAK-2 phosphorylated at Tyr570.
Application
Research Category
Signaling
Research Sub Category
Cytokines & Cytokine Receptors
This Anti-phospho JAK-2 (Tyr570) Antibody is validated for use in WB, WB, WB for the detection of phospho JAK-2 (Tyr570).
Quality
Evaluated by Western Blot in transfected HEK293T cell lysates.
Western Blot Analysis: 0.05 ug/mL of this antibody detected JAK-2 on 10 µg of transfected HEK293T cell lysates.
Target description
~ 125 kDa
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Transfected HEK293T cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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- UPC:
- 41116127
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 09-241
- Temperature Control Device:
- Yes
