ANTI-PHOSPHO-JNK1/2 (PTHR183/PTYR185) DEVELOPED IN RABBIT AFFINITY ISOLATED ANTIBODY

General description

c-Jun N-terminal kinase (JNK, SAPK) is an extensively studied memebr of the MAPK family of kinases and is critical in mediating the apoptotic signaling. There are three isoforms of JNK, JNK 1, 2 and 3. JNK 1 and 2 are ubiquitously expressed; JNK 3 is restricted to cardiac and neuronal tissues.

Immunogen

synthetic peptide derived from a region of JNK1 & 2 that contains threonine¹⁸³ and tyrosine¹⁸⁵.

Application

Anti-phospho-JNK1/2 (pThr¹⁸³/pTyr¹⁸⁵ ) antibody concentration is formulated to be used at the recommended dilutions by application.

Biochem/physiol Actions

JNK (c-Jun N-terminal kinase) is phosphorylated by MAPK (mitogen activated protein kinase) kinases 4 and 7 at Thr183 and Tyr185, leading to its activation. It is however not clear as to which MAPKK phosphorylates which amino acid residue. The activation of JNK is highly regulated and is in response to the stress signals that the cell faces. JNK phosphorylates c-Jun, the most important member of the AP-1 transcription factor family that is involved in survival, apoptosis, proliferation and tumorigenesis. JNK also activates other transcription factors such as c-myc and p53. JNK is involved in the phosphorylation of proteins such as Bad, Bcl2 and Bim that result in the execution of apoptosis by release of cytochrome c from mitochondria.
Anti-phospho-JNK1/2 (SAPK) (pThr183/pTyr185 ) specifically recognizes the endogenous, active forms of JNK1 and 2 phosphorylated at threonine 183 and tyrosine 185.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3, with 50% glycerol, 1% bovine serum albumin (BSA) (IgG and preservatives free) and 0.05% sodium azide.

Disclaimer

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