Anti-phospho-Keratin-20 Antibody (Ser13) (C15-1317-968)

General description

Keratin, type I cytoskeletal 20 (UniProt P35900; also known as CK-20, Cytokeratin-20, K20, Keratin-20, Protein IT) is encoded by the KRT20 gene (Gene ID 54474) in human. Cytokeratin-20 (Keratin-20 or K20) belongs to a family of highly conserved epithelial-specific intermediate filament (IF) proteins that play an important role in the mechanical stability and integrity of epithelial cells and tissues. Each epithelium is characterized by its unique keratin expression pattern. All epithelial cells typically express at least one type-I and one type-II keratin, which form noncovalent heteropolymers. keratinocytes preferentially express K5/K14 or K1/K10 depending on their differentiation state in the epidermis, adult hepatocytes express K8/K18 exclusively, whereas intestinal epithelial cells express K8 as the major type II keratin with varying levels of the type I K18/K19/K20, depending on the differentiation state. Keratin 20 is a type I intermediate filament protein preferentially expressed in epithelia of the stomach, intestine, uterus, and bladder, as well as in skin Merkel cells. Because of its restricted tissue distribution, K20 is widely used as a marker to help assess the origin of metastatic tumors. K20 is phosphorylated on Ser13 during cellular apoptosis induction and tissue injury. PMA treatment is shown to induce cellular K20 Ser13 phosphorylation, implicating protein kinase C (PKC) as being one of the kinases that target the phosphorylation site.

Specificity

This rabbit polyclonal antibody detected upregulated cellular Keratin-20 Ser13 phosphorylation upon phosphatase inhibitors treatment. Target site sequence is highly homologous, but not 100% conserved, among human, murine and rat species.

Immunogen

KLH-conjugated linear peptide corresponding to human Keratin-20 target region sequence containing phosphorylated Ser13.

Application

Research Category
Cell Structure

This rabbit polyclonal Anti-phospho-Keratin-20 (Ser13), Cat. No. ABT1344 is validated for use in Western Blotting for the detection of Keratin-20 Serine 13 phosphorylation.

Quality

Evaluated by Western Blotting in HeLa cells.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected upregulated Keratin-20 Ser13 phosphorylation in 10 µg lysate from phosphatase inhibitors Calyculin A- and Okadaic acid-treated HeLa cells.

Target description

~48 kDa observed. 48.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): western blot: suitable. NCBI accession no.: NP_061883. UniProt accession no.: P35900. shipped in: ambient. target post-translational modification: phosphorylation (pSer13). Gene Information: human ... KRT20(54474). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.