Anti-Phospho-PANX1 (Tyr198) (C15-1317-435)

General description

Pannexin-1 (UniProt: Q9JIP4) is encoded by the Panx1 gene (Gene ID: 55991) in murine species. Pannexin-1 is a multi-pass membrane protein of the pannexin family that serves as a high-conductance, voltage-gated channel. It is widely expressed, including in cartilage, skin, spleen, heart, and brain. In the nervous system Panx1 transcripts have been detected in the cerebellum, cortex, lens, retina, pyramidal cells, interneurons of the neocortex and hippocampus. Three pannexin family genes (PANX1-3) have been identified in murine and human genomes. Pannexins share structural and functional similarities and play important roles in several physiological processes, including ATP release, Ca2+ homeostasis, and synaptic neurotransmitters release. Pannexins can also form intermixed channels and the intermixing is more evident between Pannexin 1 and 2. The level of intermixing is governed by the degree of N-glycosylation. Post-translational N-glycosylation plays a role in cell surface targeting. Pannexin-1 can also undergo S-nitrosylation, which can inhibit channel currents and ATP release. It has been shown that Pannexin-1 channel can release ATP in response to activation by TNF-alpha that requires activation of type-1 TNF receptors and downstream signaling through Src family kinases (SFK). Tryosine 198 in the intracellular loop region of Pannexin-1 is highly conserved and is considered as a SFK phosphorylation site. Pannexin-1 has been linked to propagation of calcium waves, regulation of vascular tone, taste-bud function, and in tumor suppression in gliomas. Pannexin-1 channel opening can contribute to cell death and seizures under ischemic or epileptic conditions. (Ref.: Penuela, S et al. (2013). Biochim. Biophys. Acta 1828(11); 15-22).

Specificity

This polyclonal antibody specifically detects pannexin-1 phosphorylated on tyrosine 198 in HEK293 cells transfected with murine Pannexin 1.

Immunogen

A linear peptide corresponding to 12 amino acids surrounding phospho-Tyr198 of murine Pannexin-1.

Epitope: cytoplasmic domain

Application

Anti-Phospho-PANX1 (Tyr198), Cat. No. ABN1681, is a highly specific rabbit polyclonal antibody that targets Pannexin 1 and has been tested in Immunofluorescence and Western blotting.

Research Category
Neuroscience

Western Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in human coronary smooth muscle cells transfected with an epitope tagged Panx1, & recombinant human Pannexin1 and (active) human Src tyrosine kinase. (Courtesy of Isakson Lab, University of Virginia, Charlottesville, VA).

Immunofluorescence Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in isolated mesenteric venule cross-sections from mice (Lohman, A.W., et. al. (2015). Nat. Commun. 6:7965).

Western Blotting Analysis: A representative lot detected Phospho-PANX1 (Tyr198) in HUVECs stimulated with TNF-a (Lohman, A.W., et. al. (2015). Nat Commun. 6:7965).

Quality

Evaluated by Western Blotting in transfected HEK293 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Phospho-PANX1 (Tyr198) in 10 µg of lysate from HEK293 cells transfected with murine pannexin-1.

Target description

~58 kDa observed; 48.17 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 8.0), 0.1% BSA without azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. species reactivity (predicted by homology): rat (based on 100% sequence homology), mouse (based on 100% sequence homology). technique(s): immunofluorescence: suitable, western blot: suitable. NCBI accession no.: NP_062355. UniProt accession no.: Q9JIP4. shipped in: ambient. target post-translational modification: phosphorylation (pTyr198). Gene Information: human ... PANX1(24145). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.