ANTI-PHOSPHO-PEAK ANTIBODY (TYR665) (C15-1318-069)

General description

Pseudopodium-enriched atypical kinase 1 (Peak1; KIAA2002, or sgk269) is a ubiquitous non-receptor tyrosine kinase that belongs to the NFK3 family. It interacts with actin filaments via its N-terminal actin-targeting region. It also modulates the activity of multiple cytoskeletal regulatory proteins such as paxillin, p130Cas, Erk, and Crk; and mediates cell proliferation and migration via the Ras/Raf/Erk and Src-p130Cas-Crk-Paxillin pathways. Peak1 may play an important role in the progression of tumors.

Specificity

This antibody recognizes Peak phosphorylated at Tyr665.

Immunogen

Epitope: Phosphorylated Tyr665

KLH-conjugated linear peptide corresponding to human Peak phosphorylated at Tyr665.

Application

This phospho-Peak antibody is validated for use in WB & Dot Blot for the detection of the phospho-Peak protein.

Western Blot Analysis: A representative lot was used by an independent laboratory in 293T cells transfected with wildtype GFP-Peak. (Dr. Richard Klemke, University of California-San Diego.)

Quality

Evaluated by Dot Blot in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.

Dot Blot (Specificity) Analysis: A representative lot blocked phospho-Peak (Tyr665) in modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins.

Target description

220 kDa calculated

Analysis Note

Control
Modified and non modified peptides of phosphorylated and non-phosphorlyated Peak proteins

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): porcine (based on 100% sequence homology), rat (based on 100% sequence homology), orangutan (Sumatran. based on 100% sequence homology), mouse (based on 100% sequence homology). technique(s): dot blot: suitable, western blot: suitable. NCBI accession no.: NP_079052. UniProt accession no.: Q9H792. shipped in: wet ice. target post-translational modification: phosphorylation (pTyr665). Gene Information: human ... PEAK1(79834). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.