ANTI-PHOSPHO PKLR (SER113) 25 MIG (C15-1317-846)

General description

Mitochondrial Rho GTPase (UniProt: A1L1L6; also known as Miro1) is encoded by the Rhot1 (also known as Rnf135, rCG34120) gene (Gene ID: 303351) in rat. Miro1 is a single-pass type IV membrane protein with a transmembrane domain (aa 605-627). It functions as a mitochondrial GTPase and is involved in mitochondrial trafficking and is considered to be a potential adaptor for microtubule‐based peroxisome motility in mammalian cells. It plays a key role in mitochondrial motility on microtubules and in homeostasis. In mammals, Miro1 and Miro2 share about 60% sequence homology. Three acetylation sites for Miro1 have been identified in rat that are located on lysines 105, 525, and 629. Acetylation of Miro1 on lysine 105 (K105) is shown to increase mitochondrial transport and axon growth. Exposure of neurons to myelin-associated glycoprotein (MAG) and chondroitin sulfate proteoglycans reduces K105 acetylation of Miro1, which reduces axonal mitochondrial transport. Ac-Miro1 undergoes deacetylation with histone deacetylase 6 (HDAC6) and inhibition of HDAC6 increases Miro1 acetylation in axons. Loss of Miro1 in neurons causes depletion of mitochondria from corticospinal tract axons and leads to progressive neurological deficits similar to upper motor neuron disease. (Ref.: Kalinkski, AL., et al. (2019). J. Cell Biol. 218(6); 1871-1890; Castro, IG., et al. (2018). Traffic. 19(3); 229-242; Nguyen, TT., et al. (2014). Proc. Natl. Acad. Sci. USA. 111(35); E3631-E3640).

Specificity

This rabbit polyclonal antibody specifically detects mitochondrial Rho GTPase acetylated on lysine 105. It targets an epitope within 10 amino acids surrounding acetylated lysine 105 from the N-terminal half.

Immunogen

Linear peptide corresponding to 10 amino acids surrounding acetylated lysine 105 from the N-terminal half of rat Mitochondrial Rho GTPase (Miro1).

Application

Quality Control Testing

Evaluated by Western Blotting in Rat cortical neurons treated with Tubastatin A.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected lysine 105 acetylated Mitochondrial Rho GTPase (Miro) in lysate from rat cortical neurons treated with Tubastatin A (10 μM; 4 h)

Tested Applications

Immunofluorescence Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Immunofluorescence applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).

Western Blotting Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in Western Blotting applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).

ELISA Analysis: A representative lot detected Acetyl-Miro1 (Lys 105) in ELISA applications (Kalinski, A., et. al. (2019). J Cell Biol. 218(6):1871-1890).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Acetyl-Miro1 (Lys 105), Cat. No. ABS2247, is a rabbit polyclonal antibody that detects mitochondrial Rho GTPase (Miro) acetylated on Lysine 105 and is tested for use in ELISA, Immunofluorescence, and Western Blotting.

Physical form

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 200. conjugate: unconjugated. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. mol wt: calculated mol wt 72.16 . kDa, observed mol wt ~54 . kDa. species reactivity: rat. species reactivity (predicted by homology): mouse, human. packaging: antibody small pack of 100 . μ. g. technique(s): ELISA: suitable, immunofluorescence: suitable, western blot: suitable. isotype: IgG. UniProt accession no.: A1L1L6. shipped in: dry ice. storage temp.: 2-8°C. target post-translational modification: acetylation (Lys105). Gene Information: rat ... Rhot1(303351). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.