ANTI-PHOSPHO PKLR (SER12) 25 MIG (C005B-254259)

General description

Calsyntenin 3beta (UniProt: A0A4P8DYG6; also known as Calsyntenin-3β, CLSTN3β) is encoded by the Clstn3 (also known as Clstn3b) gene (Gene ID: 232270) in murine species. CLSTN3β is a mammal-specific that is preferentially expressed in thermogenic adipocytes and is localized in endoplasmic reticulum. Its expression in brown adipose tissue is about six-fold higher than both the inguinal subcutaneous and the perigonadal visceral white adipose tissue. However, its expression can be induced in the inguinal subcutaneous white adipose tissue upon cold exposure. Brown adipose tissue thermogenesis require innervation by the sympathetic nerves that release norepinephrine, which activates β-adrenergic receptors-cAMP-PKA pathway to drive lipolysis and thermogenic respiration. CLSTN3β is shown to promote S100b protein release that promotes this innervation and its loss is shown to reduce functional sympathetic innervation in adipose tissue. Loss of CLSTN3β also predisposes mice to obesity on a high fat diet. Clstn3β knockout mice exhibit significantly higher fasting glucose levels, impaired glucose tolerance, more lipid accumulation in brown adipose tissue, and liver steatosis. (Ref.: PluciÅ„ska, K., et al. (2020). Front. Endocrinol. 11; 579785; Zeng, X., et al. (2019). Nature. 569(7755); 229-235).

Specificity

This rabbit polyclonal antibody detects murine Calsyntenin 3b. It targets an epitope within 18 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminal region of murine Calsyntenin 3b..

Application

Quality Control Testing

Evaluated by Western Blotting in lysate from wild-type brown adipocytes.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected Calsyntenin 3b (Clstn3) in lysate from wild-type adipocytes, but not in lysate from Calsyntenin 3b knock out adipocytes.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated CLSTN3b in Immunoprecipitation applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).

Western Blotting Analysis: A representative lot detected CLSTN3b in Western Blotting applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).

Immunofluorescence Analysis: 10 ug/mL from a representative lot detected CLSTN3b in mouse primary brown adipocytes (Courtesy of Dr. Xing Zeng @UT Southwestern Medical Center).

Immunofluorescence Analysis: A representative lot detected CLSTN3b in Immunofluorescence applications (Zeng, X., et. al. (2019). Nature. 569(7755): 229-235).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CLSTN3b, Cat. No. ABS2236, is a rabbit polyclonal antibody that detects Calsyntenin 3b (Clstn3) and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Physical form

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.