Anti-phospho TIE2/TEK Antibody (Tyr992) (C15-1317-275)

General description

TIE2/TEK (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin-like loops separated by three epidermal growth factor-like repeats that are connected to three fibronectin type III-like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2/TEK are associated with inherited venous malformations; the TIE2/TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Immunogen

Epitope: Protein Kinase Domain

KLH-conjugated linear peptide corresponding to the Protein Kinase Domain of human TIE2/TEK phosphorylated at Tyr992.

Application

Immunoprecipitation Analysis: 5 µL from a representative lot immunoprecipitated phospho TIE2/TEK (Tyr992) from Pervanadate treated HUVEC cell lysate. Immunoprecipated sample was then subjected to Western Blotting using a 1:5,000 dilution of this antibody.

Peptide Inhibition Assay Analysis: A 1:500 dilution from a representative lot detected phospho TIE2/TEK (Tyr992) from transfected HEK293T lysates when pre-incubated without or with non-phospho peptide, and not when pre-incubated with phospho peptide.

Research Category
Inflammation & Immunology

Research Sub Category
Inflammation & Autoimmune Mechanisms

This Anti-phospho TIE2/TEK Antibody (Tyr992) is validated for use in western blotting, IP & peptide inhibition assay for the detection of phospho TIE2/TEK (Tyr992).

Quality

Evaluated by Western Blotting in untreated and Pervanadate treated HUVEC cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho TIE2/TEK (Tyr992) in 10 µg of Pervanadate treated HUVEC cell lysate, and not in untreated HUVEC cell lysate.

Target description

~125 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Linkage

Replaces: 09-407

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): rat (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), bovine (based on 100% sequence homology). concentration: 0.25 . mg/mL. technique(s): immunoprecipitation (IP): suitable, inhibition assay: suitable (peptide), western blot: suitable. NCBI accession no.: NP_000450. UniProt accession no.: Q02763. shipped in: wet ice. target post-translational modification: phosphorylation (pTyr992). Gene Information: human ... TEK(7010). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.